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. 2022 Jun 7;3(2):101436. doi: 10.1016/j.xpro.2022.101436

Table 1.

The mixture for phosphorylation and annealing of sgRNA oligos

Top strand for the sgRNA primer 100 pmol
Bottom strand for the sgRNA primer 100 pmol
10× T4 DNA ligase buffer 1 μL
T4 polynucleotide kinase (PNK) (10 U/ μL) 0.5 μL
ddH2O Add ddH2O to a total volume of 10 μL