Table 2.

The mixture for cloning of sgRNA DNA fragment into pX330

Annealed sgRNA mixture from Table 1	2 μL
pX330	100 ng
10× BpiI Digestion Buffer	1 μL
10 mM DTT	1 μL
10 mM ATP	1 μL
BpiI	1 μL
T4 DNA ligase (350 U/ μL)	0.5 μL
ddH2O	Add ddH2O to a total volume of 10 μL