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| Reagent | Amount |
|---|---|
| DNA template | 1 μg of genomic DNA |
| Taq DNA Polymerase | 0.5 μL |
| Primer 1 | 0.5 μL of 20 μM |
| Primer 2 | 0.5 μL of 20 μM |
| Primer 3 (optional) | 0.5 μL of 20 μM |
| PCR Buffer | 5 μL of 5× buffer |
| dNTPs | 1 μL of 10 mM |
| ddH2O | 16.5 μL |
| Total volume | 25 μL |
