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. 2022 Jun 7;3(2):101436. doi: 10.1016/j.xpro.2022.101436

Feeder cell freezing medium

Reagent Final concentration Amount
DMEM with glutamine 1× 400 mL
Fetal bovine serum Roughly 25% 100 mL
Penicillin/Streptomycin About 1% 6 mL
DMSO 10% 56.5 mL
Total n/a 562.5 mL