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ES cell growth medium
| Reagent | Final concentration | Amount |
|---|---|---|
| DMEM with glutamine | 1× | 500 mL |
| Fetal bovine serum | Roughly 15% | 75 mL |
| Non-essential amino acid | About 1% | 6 mL |
| Penicillin/Streptomycin | About 1% | 6 mL |
| β-mercaptoethanol | <0.001% | 4 μL |
| Leukemia inhibitory factor (LIF) | <0.01% | 50 μL |
| Total | n/a | 556 mL |
