ES cell freezing medium

Reagent	Final concentration	Amount
DMEM with glutamine	1×	400 mL
Fetal bovine serum	Roughly 25%	100 mL
Penicillin/Streptomycin	About 1%	6 mL
Non-essential amino acid	About 1%	6 mL
β-mercaptoethanol	<0.001%	4 μL
DMSO	10%	57 mL
Total	n/a	569 mL