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Lysis buffer for genomic DNA isolation from the ES cell culture
| Reagent | Final concentration | Amount |
|---|---|---|
| ddH2O | n/a | 89 mL |
| Tris buffer (pH = 7.6) | 20 mM | 2 mL of 1 M solution |
| NaCl | 100 mM | 2 mL of 5 M solution |
| EDTA | 10 mM | 2 mL of 0.5 M solution |
| SDS | 0.5% | 5 mL of 10% solution |
| Total | n/a | 100 mL |
