REAGENT or RESOURCE	SOURCE	IDENTIFIER
Biological samples
Pediatric female pituitary (frozen from deceased)	NIH NeuroBioBank	#1275, NDAR_INVCK755EF3
Adult female pituitary (frozen from deceased)	NIH NeuroBioBank	#5621, NDAR_INVXK717LJA
Aged female pituitary (frozen from deceased)	NIH NeuroBioBank	#187443
Pediatric male pituitary (frozen from deceased)	NIH NeuroBioBank	#1674, NDAR_INVMV348UPL
Adult male pituitary (frozen from deceased)	NIH NeuroBioBank	#5818, NDAR_INVKF350UGX
Aged male pituitary (frozen from deceased)	NIH NeuroBioBank	#187438
Chemicals, peptides, and recombinant proteins
RNAse inhibitor	New England Biolabs	# MO314L
OptiPrep	STEMCELL Technologies	07820
Sucrose	Sigma-Aldrich	S0389
EDTA	Corning	46-034-Cl
Tris-HCl, pH 7.4	Sigma-Aldrich	T2663
CaCl2	Sigma-Aldrich	21115
Magnesium acetate	Boston Bioproducts	MT-190
IGEPAL CA-630	Sigma-Aldrich	I3021
Tween-20 Surfact-Amps Detergent	Thermo Scientific	85113
Buffer EB	QIAGEN	19086
SPRI Select	Beckman Coulter	B23318
10% BSA	Sigma-Aldrich	SRE0036
PBS	Gibco	10010-023
Critical commercial assays
Chromium Single Cell 3′ Reagents, including Chips G	10× Genomics	V3, https://support.10xgenomics.com/single-cell-gene-expression/library-prep/doc/user-guide-chromium-single-cell-3-reagent-kits-user-guide-v31-chemistry
Chromium Single Cell ATAC Reagents, including Chips H	10× Genomics	V1, https://support.10xgenomics.com/single-cell-atac/library-prep/doc/user-guide-chromium-single-cell-atac-reagent-kits-user-guide-v11-chemistry
Chromium Single Cell Multiome ATAC and Gene Expression Reagent, including Chips J	10× Genomics	V1, https://support.10xgenomics.com/single-cell-multiome-atac-gex/index/doc/user-guide-chromium-next-gem-single-cell-multiome-atac-gene-expression-reagent-kits-user-guide
MiSeq Reagent Nano Kit (300 cycles)	Illumina	MS-103-1001, https://support.illumina.com/content/dam/illumina-support/documents/documentation/system_documentation/miseq/miseq-system-guide-for-local-run-manager-15027617-05.pdf
Qubit dsDNA High Sensitivity kit	Invitrogen	Q32851, https://assets.fishersci.com/TFS-Assets/LSG/manuals/Qubit_dsDNA_HS_Assay_UG.pdf
High Sensitivity DNA Bioanalyzer Kit or TapeStation	Agilent	5067-4626, https://www.agilent.com/cs/library/usermanuals/Public/G2938-90321_SensitivityDNA_KG_EN.pdf
PhiX control v3	Illumina	FC-110-3001
NovaSeq 6000 S4 Reagent Kit v1.5 (200 cycles)	Illumina	20028313
Deposited data
Raw and analyzed data	(Zhang et al., 2022)	GSE178454
Software and algorithms
Cell Ranger	10× Genomics	v5.0.0
Seurat	(Butler et al., 2018; Stuart et al., 2019)	v.3.9.9.9024; v4.0.1; v3.1.5
Cell Ranger-ATAC pipeline	10× Genomics	v1.2.0
Seurat/Signac	(Stuart et al., 2020)	v3.1.5/0.2.4
Cell Ranger ARC	10× Genomics	v1.0.0
Automated QC pipeline		This paper and https://github.com/sealfonlab/snRNA_snATAC_snMultiome_QC
Web portal	(Ruf-Zamojski et al., 2021; Zhang et al., 2022)	www.snpituitaryatlas.princeton.edu
Other
1× TE Molecular Grade	Promega	V6231
1 mL Dounce tissue glass homogenizer	VWR	# 71000-514
40 μm cell strainer	Falcon	352340
Centrifuge SW41 rotor	Beckman Coulter	#331362
Optima LE-80K ultracentrifuge	Beckman Coulter	Optima LE-80K
Cell culture centrifuge	Eppendorf	5804R
Open top thin-wall ultra clear tubes for SW41	Beckman Coulter	cat# 344059
14 mL polystyrene round-bottom tubes	Falcon	cat# 352057
Cellometer PD100 Counting Chambers	Nexcelom	CHT4-PD100-002
ViaStain Propidium Iodide (PI) staining Solution	Nexcelom	CS1-0109-5mL
Thin-wall clear 0.5-mL Qubit assay PCR tubes	Invitrogen	Q32856
Fluorometer	Invitrogen	Qubit3 or Qubit4
Bioanalyzer or TapeStation	Agilent	2100
Sequencer	Illumina	MiSeq
Sequencer	Illumina	NovaSeq 6000
Cell counter	Nexcelom	Cellometer K2, https://www.nexcelom.com/knowledge-base/cellometer-k2-user-manaul/
Falcon 6-well clear flat bottom cell culture plate	Corning	Cat# 353046
HEPES buffer, 1 M	Corning	Cat# 255-060-CL
Magnesium chloride 1 M	Sigma-Aldrich	Cat# M1028
KCl	Fisher Scientific	Cat# P217-3
Glycerol	Sigma-Aldrich	Cat# G6279
Nuclei preservation protocol	Collas Lab, University of Oslo	http://collaslab.org/wp-content/uploads/2011/07/Isolation_somatic_cell_nuclei.pdf