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. 2022 Jun 8;221(7):e202108015. doi: 10.1083/jcb.202108015

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© 2022 Wang et al.

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Figure 3. — ORF10 impairs cilium biogenesis and maintenance. (A) ORF10 inhibits cilium biogenesis. Immunofluorescence analysis of Ac-Tubulin (green) and HA (red) in ORF10-expressing and empty vector control MRC-5 cells. Nuclei were stained with DAPI (blue). (B) Quantification of the distribution of ciliated cells among the ORF10-expressing and empty-vector control MRC-5 cells (A; n = 5 independent experiments). Data are presented as the mean ± SEM. *** indicates P < 0.001. Statistical analysis was performed with two-tailed unpaired Student’s t test. (C) ORF10 perturbs cilium maintenance. Immunofluorescence analysis of Ac-Tubulin (green) and HA (red) in ORF10-expressing and empty-vector control MRC-5 ciliated cells. Nuclei were stained with DAPI (blue). (D) Quantification of the ratio of ciliated cells in ORF10 expressed and empty-vector transfected ciliated cells (C;n = 5 independent experiments). Data are presented as the mean ± SEM. *** indicates P < 0.001. Statistical analysis was performed with two-tailed unpaired Student’s t test. Source data are available for this figure: SourceData F3.