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. 2022 May 30;49(2):117–126. doi: 10.5653/cerm.2021.05120

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Copyright © 2022. THE KOREAN SOCIETY FOR REPRODUCTIVE MEDICINE

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Quantitative intracellular H₂O₂ generation was evaluated by the measurement of dichlorofluorescein (DCF) fluorescence intensity using imaging-flow cytometry. Flow-cytometric histograms show the amount of intracellular H₂O₂ generation H₂O₂ in sperm. (A) Fresh nonfrozen group, (B) SuV group, (C) SuV+3A group, (D) Vapor group, and (E) Vapor+3A group. Values are presented as mean±standard error of the mean. Significant differences were defined as p-values less than 0.05. SuV, sucrose vitrification; 3A, triple antioxidants; Vapor, liquid nitrogen vapor freezing.