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. 2022 May 21;28:920–934. doi: 10.1016/j.omtn.2022.05.033

Figure 1.

Figure 1

Overexpression of Rps4l in vivo and in vitro inhibits hypoxia-induced pyroptosis

(A) Electron micrographs of PASMCs of lung tissues in Rps4lTg and wild-type (WT) hypoxic and normoxic mice. The arrow points to the location of the cell membrane swelling and breakage (down, scale bar, 1 μm). Immunofluorescence analysis of (B) c-caspase-1, (C) NLRP3, (D) ASC in the lung tissues of Rps4lTg and WT hypoxic and normoxic mice (scale bar, 25 μm). Western blotting analysis of (E) c-caspase-1, NLRP3, ASC, IL-1β, and IL-18 in the lung tissues of Rps4lTg and WT hypoxic and normoxic mice. (F) Electron micrographs of hypoxic and normoxic PASMCs. The arrow points to the location of the cell membrane swelling and breakage (right, scale bar, 1 μm; left, scale bar, 2 μm). (G) LDH release assay in hypoxic and normoxic PASMCs transfected with OE-Rps4l or OE-NC. (H) PI staining in hypoxic and normoxic PASMCs transfected with OE-Rps4l or OE-NC (scale bar, 50 μm). (I) Flow cytometry analysis of hypoxic and normoxic PASMCs transfected with OE-Rps4l or OE-NC. All values are represented as the mean ± SEM (∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001; n ≥ 3). NOR, normoxia; HYP, hypoxia.