Skip to main content
. 2022 May 21;28:920–934. doi: 10.1016/j.omtn.2022.05.033

Figure 2.

Figure 2

lnc-Rps4l-encoded peptide RPS4XL inhibits hypoxia-induced pyroptosis of PASMCs

(A) Western blotting analysis of RPS4XL in hypoxic and control PASMCs transfected with OE-Rps4l, Mut-Rps4l, or OE-NC. (B) LDH release assay using hypoxic and control PASMCs transfected with OE-Rps4l, Mut-Rps4l, or OE-NC. (C) PI staining in hypoxic and control PASMCs transfected with OE-Rps4l, Mut-Rps4l, or OE-NC (scale bar, 50 μm). Western blotting analysis of (D) c-caspase-1, (E) NLRP3, ASC, IL-1β, and IL-18 in hypoxic and normoxic PASMCs transfected with OE-Rps4l, Mut-Rps4l, or OE-NC. (F) Flow cytometry analysis in hypoxic and control PASMCs transfected with OE-Rps4l, Mut-Rps4l, or OE-NC. All values are represented as the mean ± SEM (∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001; n ≥ 3). NOR, normoxia; HYP, hypoxia.