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. 2022 May 21;28:920–934. doi: 10.1016/j.omtn.2022.05.033

Figure 5.

Figure 5

The functional domain of RPS4XL

(A) Schematic diagram of the vector construction with the different RPS4XL domains. SWISS-MODEL schematic structural model of the different RPS4XL domains. (B) HSC70 protein expression in hypoxic and normoxic PASMCs overexpressing RPS4XL 1–41 aa, 42–104 aa, and 105–262 aa domains. (C) PI staining in hypoxic and normoxic PASMCs overexpressing the RPS4XL 1–41 aa, 42–104 aa, and 105–262 aa domains (scale bar, 100 μm). (D) LDH release assay in hypoxic and normoxic PASMCs overexpressing the RPS4XL 1–41 aa, 42–104 aa, and 105–262 aa domains. Western blotting analysis of (E) c-caspase-1, (F) NLRP3, ASC, IL-1β, and IL-18 in hypoxic and normoxic PASMCs overexpressing the RPS4XL 1–41 aa, 42–104 aa, and 105–262 aa domains. All values are represented as the mean ± SEM (∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001; n ≥ 3). NOR, normoxia; HYP, hypoxia; NS, no significance.