Table 3.
Primers and their sequences for PCR analysis
| Primer | Sequence (5’-3’) |
|---|---|
| GAPDH-F | GGAAGCTTGTCATCAATGGAAATC |
| GAPDH-R | TGATGACCCTTTTGGCTCCC |
| CCR7-F | TGAGGTCACGGACGATTACAT |
| CCR7-R | GTAGGCCCACGAAACAAATGAT |
| CD40LG-F | ACATACAACCAAACTTCTCCCCG |
| CD40LG-R | GCAAAAAGTGCTGACCCAATCA |
| IL-4-F | CCAACTGCTTCCCCCTCTG |
| IL-4-R | TCTGTTACGGTCAACTCGGTG |
| CD80-F | GGCCCGAGTACAAGAACCG |
| CD80-R | TCGTATGTGCCCTCGTCAGAT |
| IL-10-F | TACCACCTCCCGAAAATGTCA |
| IL-10-R | CCCAGTCTGAATGCTCATCTG |
| CTLA-4-F | GCCCTGCACTCTCCTGTTTTT |
| CTLA-4-R | GGTTGCCGCACAGACTTCA |
| CD86-F | CTGCTCATCTATACACGGTTACC |
| CD86-F | GGAAACGTCGTACAGTTCTGTG |
| IL-2-F | ACCGCTTTGCGGAATCTCA |
| IL-2-R | AGGTCAGGGAAACATCAGGGA |
| IL-6-F | ACTCACCTCTTCAGAACGAATTG |
| IL-6-R | CCATCTTTGGAAGGTTCAGGTTG |
| STAT3-F | CAGCAGCTTGACACACGGTA |
| STAT3-R | AAACACCAAAGTGGCATGTGA |