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. 2022 May 15;14(5):3066–3078.

Figure 5.

Figure 5

HC030031 reduced cell proliferation, migration, and co-localization of TRPV1 and TRPA1. Co-culture of macrophages derived from peritoneal lavage fluid and ectopic endometrial hEM15A cells treated with/without TRPA1 antagonist (HC030031). A. Cell proliferation was assayed by EDU staining. B. Transwell assay was performed to evaluate cell migration. C. The percentage of cell proliferation was indicated by EDU positive cells. D. Migrated cells stained by crystal violet indicated by the optical density of crystal violet (OD570 nm). **denotes P<0.01, compared with Normal group; ##denotes P<0.01, compared with (-) HC-030031 group. n=3. E. After being treated with HC030031, the co-localization of TRPV1 and TRPA1 was detected by immunofluorescence.