TABLE 2.
Osmoprotectant | % Inhibition by:
|
|||
---|---|---|---|---|
Glycine betaine | l-Carnitine | d-Carnitine | Choline | |
l-[14C]carnitine | 70 | 85 | 89 | 59 |
[14C]glycine betaine | 89 | 2 | 3 | 25 |
Bacteria were grown in DM. Uptake assays were carried out as described in the text with either [14C]glycine betaine or l-[14C]carnitine (10 μM). Unlabeled competitors were added in 100-fold excesses. Results are given as the percent reductions of the uninhibited uptake rates, which were 2.0 and 3.4 nmol/min/mg (dry weight) for l-carnitine and glycine betaine, respectively. The results are the means of three independent experiments. The standard deviations were less than 5%.