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. 2022 May 15;12(5):2012–2031.

Figure 3.

Figure 3

hsa_circ_0001741 promotes ESCC cell stemness, invasion, and migration. (A, B) Representative image showing tumorsphere formation (A) after knockdown hsa_circ_0001741 in TE-1 cells and (B) after overexpression of hsa_circ_0001741 in ECA109 cells. (C, D) The effect of hsa_circ_0001741 on ESCC cells invasion and migration abilities using transwell assay (C) after knockdown of hsa_circ_0001741 in TE1 cells and (D) after overexpression of hsa_circ_0001741 in ECA109 cells. (E) The expression of hsa_circ_0001741 in TE1 cell line transfected with siRNAs or si-NC measured by qRT-PCR. (F) The overexpression plasmid of hsa_circ_0001741 or the control vector was transfected into ECA109 cell line, and the expression level of hsa_circ_0001741 was measured by qRT-PCR. (G, H) Cancer stemness and EMT marker protein levels in ESCC cells (G) after hsa_circ_0001741 knockdown or (H) after hsa_circ_0001741 overexpression was detected by Western blot. **P < 0.01, ***P < 0.001.