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. 2022 May 15;12(5):2012–2031.

Figure 6.

Figure 6

NOTCH3 reverses the function of hsa_circ_0001741 in ESCC cells. (A, B) The cancer stemness (A) and invasion and migration (B) abilities in TE1 cells inhibited by hsa_circ_0001741 knockdown were reversed after transfection with NOTCH3 overexpression plasmid using tumorsphere formation and transwell assay. (C, D) The cancer stemness (C) and invasion and migration (D) abilities promoted by hsa_circ_0001741 overexpression in ECA109 cells were reversed after transfection with miR-491-5p mimics using tumorsphere formation and transwell assay. (E) The downregulation of NOTCH3, SOX2, Nanog, N-cadherin, and the upregulation of E-cadherin induced by hsa_circ_0001741 knockdown in TE-1 was reversed by overexpression of NOTCH3 as detected by Western blotting. (F) The upregulation of NOTCH3, SOX2, Nanog, N-cadherin, and the downregulation of E-cadherin induced by hsa_circ_0001741 overexpression in ECA109 were abolished by knockdown NOTCH3 and detected by Western blotting. **P < 0.01, ***P < 0.001.