Figure 7.

circ_6790 regulates the expression of S100A11 through CBX7. (A) Differentially expressed genes in PDAC that were significantly and negatively associated with CBX7. (B) ChIP-seq database analysis of the binding of CBX7 to the S100A11 promoter. (C) Correlation between S100A11 expression and survival of PDAC patients. (D) Correlation between the expression of S100A11 and the level of immune cell infiltration in PDAC tissues. (E, F) Detection of S100A11 expression in PDAC cells and hTERT-HPNE cells by RT-qPCR (E) and western blot (F). (G, H) Detection of S100A11 expression in PDAC cells under different treatments by RT-qPCR (G) and western blot (H). (I) Co-IP detection of the binding relationship between CBX7 and DNMTs. (J) The enrichment ability of CBX7 and DNMTs for S100A11 promoter in each group of cells examined using ChIP-qPCR. (K) CpG site on S100A11 promoter. (L) The DNA methylation level of S100A11 promoter in each group of cells examined using MSP. *P < 0.05. Data are shown as mean ± SD of three technical replicates. One-way (E, F) or two-way ANOVA (G, H, J) were used for data comparison.