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. 2022 Jun 2;26(1):246. doi: 10.3892/mmr.2022.12762

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Copyright: © Hu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — LINC00961 knockdown attenuates TGF-β-induced EndMT. (A) Immunofluorescence staining was used to identify CD31⁺/α-SMA⁺ cells. α-SMA (red), CD31 (green); scale bars: 50 µm. (B) Reverse transcription-quantitative PCR was used to evaluate EndMT-related mRNA. (C) Western blotting was used to evaluate EndMT-related proteins. (a) Control, (b) TGF-β, (c) TGF-β + sh-LINC00961, (d) TGF-β + sh-Scramble, (e) TGF-β + LV-LINC00961 and (f) TGF-β + LV-Control. Each experiment was repeated at least three times, ns, P>0.05; *P<0.05 and ^#P<0.05. n=5 per group. EndMT, endothelial-mesenchymal transition; α-SMA, α-smooth muscle actin; sh-, short hairpin; LV, lentivirus; VE-Cad, VE cadherin; FSP-1, fibroblast specific protein 1; ns, not significant.