Table II.
Information on primary and secondary antibodies.
| Antibodies | Species | Supplier | Catalogue number | Dilution |
|---|---|---|---|---|
| Primary | ||||
| GAPDH | Rabbit | Abcam | ab37168 | 1:10,000 |
| PTEN | Rabbit | Abcam | ab267787 | 1:2,000 |
| p-PI3k | Rabbit | Abcam | ab182651 | 1:500 |
| PI3k | Rabbit | Cell Signaling Technology, Inc. | 4292 | 1:3,000 |
| p-AKT | Rabbit | Cell Signaling Technology, Inc. | 4060 | 1:1,000 |
| AKT | Rabbit | Cell Signaling Technology, Inc. | 9272 | 1:2,000 |
| SPARR | Rabbit | Cell Signaling Technology, Inc. | 25823 | 1:1,000 |
| p-mTOR | Rabbit | Cell Signaling Technology, Inc. | 5536 | 1:500 |
| mTOR | Rabbit | Cell Signaling Technology, Inc. | 2972 | 1:1,000 |
| CD31 | Mouse | Santa Cruz Biotechnology, Inc. | sc-376764 | 1:500 |
| VE-Cadherin | Rabbit | Thermo Fisher Scientific, Inc. | 36-1900 | 1:500 |
| α-SMA | Rabbit | ProteinTech Group, Inc. | 14395-1-AP | 1:5,000 |
| FSP-1 | Rabbit | ProteinTech Group, Inc. | 20886-1-AP | 1:1,000 |
| Cyclin D1 | Rabbit | Cell Signaling Technology, Inc. | 55506 | 1:1,000 |
| Bcl-2 | Rabbit | Abcam | ab59348 | 1:1,000 |
| Bax | Rabbit | Cell Signaling Technology, Inc. | 2772 | 1:2,000 |
| Secondary | ||||
| HRP-goat anti-rabbit | Aspen Biotechnology Co., Ltd. | AS1107 | 1:10,000 | |
| HRP-goat anti-mouse | Aspen Biotechnology Co., Ltd. | AS1106 | 1:10,000 |
p-, phosphorylated; α-SMA, α-smooth muscle actin; FSP-1, fibroblast specific protein 1; PTEN, phosphatase and tensin homolog deleted on chromosome 10; HRP, horseradish peroxidase.