TABLE 1.
Oxidation of 12 PAH by laccase from T. versicolor alone and in the presence of HBA or HBT
| PAH (5 μM) | Oxidation (% of control without enzyme) witha:
|
||
|---|---|---|---|
| Laccase | Laccase + HBA | Laccase + HBT | |
| Naphthalene | 0.7 ± 2.5 | 0.0 ± 1.9 | 0.0 ± 2.1 |
| Acenaphthylene | 7.5 ± 1.4 | 93.7 ± 1.3 | 97.5 ± 0.1 |
| Acenaphthene | 6.9 ± 2.5 | 96.7 ± 1.7 | 100.0 ± 0.0 |
| Fluorene | 1.8 ± 1.7 | 83.9 ± 4.9 | 95.3 ± 0.2 |
| Phenanthrene | 0.0 ± 1.1 | 0.0 ± 1.6 | 7.3 ± 2.7 |
| Anthracene | 73.8 ± 1.2 | 83.9 ± 0.9 | 84.0 ± 0.4 |
| Fluoranthene | 0.0 ± 1.9 | 0.6 ± 2.2 | 6.8 ± 1.2 |
| Pyrene | 0.0 ± 1.6 | 19.6 ± 5.0 | 86.5 ± 1.4 |
| Chrysene | 0.0 ± 2.2 | 0.7 ± 2.8 | 0.0 ± 2.0 |
| Benzo[k]fluoranthene | 0.0 ± 1.9 | 5.4 ± 4.6 | 2.1 ± 5.4 |
| Benzo[a]pyrene | 5.3 ± 1.7 | 52.9 ± 4.9 | 62.0 ± 2.1 |
| Perylene | 0.0 ± 1.4 | 79.9 ± 4.9 | 87.8 ± 2.8 |
a
Laccase was at 4 U/ml; HBA and HBT were at 1 mM. Results are means and standard deviations.