TABLE 2.
Oxidation of PAH by laccase from T. versicolor in the presence of amino acids and derivatives
| Amino acid or derivative (1 mM) | Oxidation (% of control) ofa:
|
|||
|---|---|---|---|---|
| Acenaphthylene | Acenaphthene | Fluorene | Anthracene | |
| None | 42.5 ± 0.1 | 20.1 ± 0.1 | 2.6 ± 0.3 | 25.7 ± 0.6 |
| Methionine | 47.3 ± 3.5 | 8.2 ± 4.2 | 4.9 ± 1.9 | 33.2 ± 3.3 |
| Cysteine | 95.3 ± 0.9 | 5.9 ± 4.1 | 0.1 ± 2.6 | 19.9 ± 4.8 |
| Tyrosine | 97.9 ± 0.3 | 42.5 ± 5.0 | 18.3 ± 6.4 | 44.2 ± 6.2 |
| Tyrosine hydrazide | 97.9 ± 0.1 | 41.0 ± 4.9 | 31.8 ± 2.5 | 63.0 ± 5.9 |
| Reduced glutathione | 99.2 ± 0.1 | 15.0 ± 2.2 | 3.6 ± 2.0 | 34.1 ± 1.9 |
a
Each PAH was at 25 μM; laccase was at 4 U/ml. Results are means and standard deviations.