Fig. 2.

The analysis of ORFs in circular RNAs. The possible ORF organization were illustrated. The ORFs not crossing backsplicing junction were denoted ‘0−crossing’ (left). ORFs crossing backsplicing junctions (once) toward 5’ or 3’ will generate novel N terminal or C terminal sequences. 2C denoted the ORFs crossing backsplicing junction twice. In some cases, circRNA generates ORF crossing backsplicing junction infinite times. Grey circle denotes the absence of a feature (novel sequence or infinite ORF) while green circle denotes the presence of the feature