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. 2022 Jun 10;20:268. doi: 10.1186/s12951-022-01485-8

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Evaluation of RWM penetration and in vivo release profile. A Fluorescent images of NiRe-PPS-NP penetrating three layers (OE, CT and IE) of round window membrane in control, 0.5, 2 and 6 h time points. B MFI of Nile Red along the RWM from middle ear side to scala tympani side. C ATX concentrations in control cochlear perilymph in various time points (0.5, 2, 6 and 12 h) and CDDP-treated ones (2 h). ^*** p < 0.001