Fig. 8.

Chlorogenic acid decreases inflammation, oxidative stress of primary cortical neurons induced by oxygen and glucose deprivation. a Primary cortical neurons, treated with different concentrations of CGA without OGD; cell viability was assessed by CCK8. n = 3. b Primary cortical neurons pretreated with different concentrations of CGA before OGD; cell viability was assessed by CCK8. n = 3. c The effect of Sirt1 inhibitor, EX-527 on neuronal viability 24 h after OGD for 2 h. n = 3. d Western blot evaluation of the protein levels of iNOS, SOD2/MnSOD, TNF-α, and pre-IL-1β and mature- IL-1β 24h after OGD. e–h Quantification of western blot data of iNOS, SOD2/MnSOD, TNF-α, and mature- IL-1β.∗∗P < 0.01 and ∗∗∗P < 0.001 vs. the control group. #P < 0.05 and ###P < 0.001 vs. the OGD group. n = 3