Figure 5.
M1 and M2b macrophages have compromised effects on the cell growth, invasion, and migration of cocultured PDAC cells. The effects of differently polarized macrophages on the cell growth, invasion, and migration of cocultured PDAC cells were examined in a coculture system. (A) CCK-8 assay for PANC-1 cells. (B,C) Transwell cell invasion assay (the upper chamber was filled with 2×105 PANC-1 cells with differentially polarized macrophages or PANC-1 cells alone), shown by quantification (B) and by representative images stained with 0.1% crystal violet (C). (D,E) Transwell cell migration assay (the upper chamber was filled with 2×105 PANC-1 cells with differentially polarized macrophages or PANC-1 cells alone), shown by quantification (D) and by representative images stained with 0.1% crystal violet (E). *, P<0.05 (blue *: M2b vs. M2a or M2c or M2d). N=5. Scale bars were 100 µm. Mϕ: macrophages. PDAC, pancreatic ductal adenocarcinoma; CCK-8, Cell Counting Kit-8.