FIG. 2.

Analysis of an artificial rDNA mixture by competitive clamping: schematic diagram of PCR clamping performed with PNA-ALF and results of dot blot hybridizations with polynucleotide probes specific for SJA-53 and SJA-186. Positions P1 to K3, selected clones from libraries A1 to A3 (PCR clamping); positions L3 to G5, selected clones from libraries A4 to A6 (control PCR). The following 16S rDNAs were used as controls: SJA-9 (position I5), SJA-53 (position J5), SJA-105 (position K5), and SJA-186 (position M5). Asterisks indicate positions where no samples were applied.