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. Author manuscript; available in PMC: 2022 Nov 15.
Published in final edited form as: Nature. 2022 May 11;605(7910):527–531. doi: 10.1038/s41586-022-04717-x

Extended Data Fig 3. |. Lytic activity, SpeB production, and binding of GAS strains.

Extended Data Fig 3. |

a,b, Keratinocytes were infected (MOI=100) with GAS 5448, isogenic mutant controls, or clinical isolates for 4 h and (a) lysis measured by LDH release and (b) SpeB activity measured using specific substrate Mca-IFFDTWDNE-Lys-Dnp. c, GAS labeled with Bocillin was incubated with keratinocytes (MOI=100) 1 h, washed, and adherence measured by fluorescence. Data are representative of three independent experiments with 3 technical replicates and are presented as the mean±s.d. P values were calculated by two-way ANOVA compared to 5448 (M1) control; P<0.0001.