TABLE 2.

Primary sequences and physicochemical properties of Library 2 peptides, which correspond to W-position II shown in Figures 1D–F.

Name	Trp (W)	Sequence	Length (r)	Chargea	H	μH
E24	3	VW RR WR RR WR	10	6	0.191	0.901
E61	3	WV RR WR RR VR RW	12	7	0.177	0.89
E62	3	VW RR WR RV WR RV RR	14	8	0.166	0.868
E65	3	VW RR VR RV WR RV RR RW	16	9	0.159	0.827
E67	3	VW RR VR RV WR RV RR VW RR	18	10	0.153	0.818
E68	3	RW VR RV RR WV RR VR RW VR RV	20	11	0.148	0.82
E69	3	RV WR RV RR VW RR VR RV WR RV	20	11	0.148	0.822
E70	3	RW VR RV RR WV RR VR RV VR RW	20	11	0.148	0.844
E71	3	RR VW RR VR RV WR RV RR VV RR VW	22	12	0.144	0.777
E73	3	RR WV RR VR RW VR RV RR VV RR WV	22	12	0.144	0.814
E74	3	RR WV RR VR RW VR RV RR WV RR VV	22	12	0.144	0.791
E75	3	RR VW RR VR RV WR RV RR VW RR VV	22	12	0.144	0.798
E77	4	RR WW RR WV RW	10	5	0.517	0.938
E31	4	RR WW RV WR RV WR	12	6	0.448	0.963
E78	4	VW RR WR RR WR RW	12	7	0.263	0.963
E79	4	RR WV RW VR RW VR RW	14	7	0.399	0.942
E80	4	RR WV RW VR RW VR WV RR	16	8	0.363	0.85
E81	4	RR WV RW VR RW VR RV RR WV	18	9	0.334	0.365
E82	4	RR VW RV WR RV WR VV RR VW RR	20	10	0.311	0.811
E85	5	RR WW RV WR RW WR	12	6	0.534	1.021
E88	5	RR WV RW WR RW VR RW	14	7	0.473	1.009
E93	5	RR WV RW VR RW VR WW RR	16	8	0.427	0.911
E95	5	RR VW RV WR RV WR RW RR VW	18	9	0.391	0.921

H, hydrophobicity and μH, hydrophobic moment were determined using the online peptide modeling software heliquest.ipmc.cnrs.fr/; peptides of the same length are minor positional variants of W; r, residues

The sequences were modeled as idealized amphipathic helices based on helical wheel diagrams using the online modeling software Heliquest, which also provides the physicochemical properties listed in the table.

^aAll charges are positive.