FIG. 7.

Localization of the S. hyicus lipase in strain JIM5496 (P23) by Western blotting with anti-CtermLIP sera. Panel 1, total cell extract; panel 2, sample without treatment; panel 3, sample incubated for 30 min at 37°C; panel 4, cell wall and protoplast fractions incubated with trypsin for 30 min at 37°C; panel 5, protoplasts incubated with Triton X-100 and trypsin for 30 min at 37°C; panel 6, total cell extract incubated with Triton X-100 for 30 min at 37°C. Abbreviations: T, total cell extract; W, cell wall fraction; C, cytoplasm fraction; M, membrane fraction; CM, protoplasts; Tryp, trypsin; TX100, Triton X-100.