FIG. 4.
Agar plate mutagenicity test with V. harveyi BB7, BB7X, BB7M, and BB7XM. V. harveyi cells (4 × 106) grown in BOSS medium were spread on BOSS plates containing mutagens; after incubation for 48 h at 30°C, the number of neomycin-resistant mutants was counted and the percentage of mutants was calculated. Ctrl, control experiments (without any mutagen). The following amounts of mutagens were added per plate: daunomycin (Dau), 6 μg/ml; sodium azide (SA), 1.5 μg/ml; 2-methoxy-6-chloro-9-(3-(2-chloroethyl)aminopropylamino)acridine · 2HCl (ICR-191), 1 μg/ml; 4-nitro-o-phenylenediamine (NPD), 20 μg/ml; 4-nitroquinolone-N-oxide (NQNO), 0.5 μg/ml; methyl methanesulfonate (MMS), 1 μg/ml; and 2-aminofluorene (2-AF), 10 μg/ml. The results presented are mean values from two independent experiments.