TABLE 1.

Medium composition used for the different fed-batch fermentations^a

Fermentation no., feeding rate, and feeding time	Initial concn in fermentation medium (g liter−1)		Concn in feeding solution (g liter−1)b
	Glucose	CNSc	Glucose	CNSc
1, constant at 0.13 liters/h for 30.5 h	2	2	120	264
2, constant at 0.15 liters/h for 27.0 h	2	112	120
3, constant at 0.14 liters/h for 28.5 h	57	2		264
4, constant at 0.36 liters/h for 11.0 h	2	2	120	264
5, constant at 0.06 liters/h for 72.0 h	2	2	120	264
6, acidification-controlled for 12 h	11	25	120	264

^aFermentations were started with an initial volume of 8 liters of modified MRS medium; an additional 4 liters of a concentrated solution of glucose and/or CNS was added during fed-batch fermentation. 

^bThis feeding would result in a medium with final concentrations of 40 g of glucose per liter and 88 g of CNS per liter in a volume of 12 liters. 

^cCNS contained the following (in grams per gram): Lab Lemco powder (Oxoid), 0.36; yeast extract (Merck), 0.18; bacteriological peptone (Oxoid), 0.46.