Figure 3.

Metformin and DQ alone or with each other improved healthy ovarian reserve and function in cisplatin-treated mice. (a, h) Representative images of Sirius red stained ovaries and quantitative analysis of Sirius red positive area. Scar bars were marked on respective images (n =3). (b, c) HE staining of ovarian sections and quantitative counting of follicles at different levels (n =5). Black arrow: primordial follicle; red arrow: primary follicle, secondary follicle and antral follicle; yellow arrow: atretic follicle. (d) Regular and irregular estrous cycle percentage of different medication groups (n =6). (e) Mice ovarian index in different medication groups (n =10). (f, g) Serum AMH (n =8) and E₂ (n =7) of following the administration of different drugs. Results are presented using mean ± standard deviation values. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.