Figure 5.

Metformin and DQ alleviate cisplatin-induced ovarian DNA damage. (a, d) Representative images of 8-OHdG immunohistochemical staining after different medications and quantitative analysis of 8-OHdG–positive expression based on IOD (n =3). (b, c, e, f) TUNEL and EdU fluorescence of ovarian sections after different medications and determination of TUNEL- and EdU-positive cell numbers (n =3). (g, h) Western blot analysis of SOD2, HO1, and PCNA protein expressions in ovaries treated with different medication regimens (n =3). Results are presented using mean ± standard deviation values. ∗∗p < 0.01, ∗∗∗p < 0.001.