Fig. 4.

Evaluation of calcium involvement in melatonin antitumor effects. (a) Treatment of sarcoma cell lines with calcium chloride abolishes the antiproliferative effect induced by melatonin treatment after 48 h (b) while BAPTA treatment potentiates such inhibition. (c) Microscopic images taken with 10 × objective show that calcium chloride supplementation abolishes the effects of melatonin on cell migration. Graphs represent the mean wound healing size (µm) of each experimental group. (d) Combined treatment of melatonin and calcium chloride for 8 h reverses the observed melatonin effects on N-cadherin and vimentin expression in sw-1353 cells. Optical density reading values versus loading control GAPDH are presented. Dashed line indicates control group levels. * p ≤ 0.05 vs control group (vehicle-treated cells). # p ≤ 0.05 vs melatonin group