Figure 3.

Systems biology changes when taking AX. A) C-means clusters from all omics that follow a dose-response and reverse dose-response. Select pathways and features of interest are displayed for each. Individual analytes tested for significance using multiple linear regression. B) LDL trend for all three fibers over the course of supplementation. Both AX and mix are significant (one-way ANOVA). Data are normalized to baseline and then raised to the mean of the cohort. C) The longitudinal response of the bile acids UDCA, CDCA and LCA over the course of fiber supplementation for all three fibers. Significance determined by one-way ANOVA. D) Significant correlations between bile acids (y-axis) and microbial taxa (x-axis) represented by bubble plot. Bubbles are colored by direction of correlation coefficient and the bubble size is represented by − log₁₀(p-value). P-values determined by Spearman correlations, FDR corrected E) CEs that significantly reduce in a dose-response manner with AX supplementation. Significance determined by one-way ANOVA. F) Correlation network of lipids, bile acids and clinical cholesterol levels, where negative correlations are connected with a red edge and positive correlations are connected with a blue edge. Orange node = bile acid, yellow node = lipid, and blue node = clinical lipid value. All edges are spearman correlations with FDR < 0.05. See also Figure S2 and S3; and see Tables S1C, S2D, 3C, 3D and S4A.