Skip to main content
. Author manuscript; available in PMC: 2023 Jun 8.
Published in final edited form as: Cell Host Microbe. 2022 Apr 11;30(6):786–797.e8. doi: 10.1016/j.chom.2022.03.015

Key resources table.

REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Anti-mouse CD45 Pacific Blue BioLegend Cat#103125
Anti-mouse CD3 FITC BioLegend Cat#100306
Anti-mouse CD4 APC Cy7 BioLegend Cat#100414
Anti-mouse CD19 PerCP Cy5.5 eBioscience Cat#45019842
Anti-mouse Tbet APC BioLegend Cat#644814
Anti-mouse GATA3 PE eBioscience Cat#12698180
Anti-mouse FoxP3 PE Cy7 BioLegend Cat#137235
Anti-mouse IFN AF700 BioLegend Cat#505824
Bacterial and virus strains
E. coli: BW25113: F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514 National BioResource Project at the National Institute of Genetics, Japan (Baba et al., 2006) N/A
E. coli: GC1547: BW25113 transformed with pET28 this study N/A
E. coli: GC1549: BW25113 ΔfabF::kan this study N/A
E. coli: GC1558: ΔeutN::kan, pGC735 this study N/A
E. coli: GC1559: ΔfabH::kan, pGC738 this study N/A
E. coli: GC1643: ΔeutC::kan this study N/A
E. coli: GC1644: BW25113 ΔeutC this study N/A
E. coli: GC1645: BW25113 ΔeutN::kan this study N/A
E. coli: GC1646: BW25113 ΔeutN this study N/A
E. coli: GC1647: BW25113 ΔeutC ΔeutN::kan this study N/A
E. coli: GC1648: BW25113 ΔeutC::kan ΔeutN this study N/A
E. coli: GC1649: BW25113 ΔeutD this study N/A
E. coli: GC1650: BW25113 ΔeutD pPlac-eutD this study N/A
E. coli: JW1077: BW25113 ΔfabH::kan National BioResource Project at the National Institute of Genetics, Japan (Baba et al., 2006) N/A
E. coli: JW2442: BW25113 ΔeutD::kan National BioResource Project at the National Institute of Genetics, Japan (Baba et al., 2006) N/A
E. coli: JW2440: BW25113 ΔeutN::kan National BioResource Project at the National Institute of Genetics, Japan (Baba et al., 2006) N/A
E. coli: JW2294: BW25113 Δpta::kan National BioResource Project at the National Institute of Genetics, Japan (Baba et al., 2006) N/A
E. coli: JW3595: BW25113 ΔrfaF::kan National BioResource Project at the National Institute of Genetics, Japan (Baba et al., 2006) N/A
E. coli: JW3818: BW25113 ΔrfaH::kan National BioResource Project at the National Institute of Genetics, Japan (Baba et al., 2006) N/A
E. coli: JW4334: BW25113 ΔholD::kan National BioResource Project at the National Institute of Genetics, Japan (Baba et al., 2006) N/A
E. coli: JW1859: BW25113 ΔcmoA::kan National BioResource Project at the National Institute of Genetics, Japan (Baba et al., 2006) N/A
E. coli: JW3397: BW25113 ΔyhgN::kan National BioResource Project at the National Institute of Genetics, Japan (Baba et al., 2006) N/A
E. coli: JW3929: BW25113 ΔargE::kan National BioResource Project at the National Institute of Genetics, Japan (Baba et al., 2006) N/A
E. coli: JW3932: BW25113 ΔargH::kan National BioResource Project at the National Institute of Genetics, Japan (Baba et al., 2006) N/A
Chemicals, peptides, and recombinant proteins
L-arginine monohydrochloride Sigma-Aldrich Cat#A5131
L-ornithine monohydrochloride Sigma-Aldrich Cat#O2375
Acetaldehyde Chem Service Cat#S-11005A4
Ethanolamine Sigma-Aldrich Cat#E0135
N-acetyl cysteine Sigma-Aldrich Cat#A9165
Roche Diagnostics LIGHTCYCLER 480 SYBR GREEN Roche Cat#04887352001
Critical commercial assays
RNeasy Plus Mini Kit QIAGEN Cat#74136
QIAprep Spin Miniprep Kit
QIAEX II Gell Extraction Kit QIAGEN Cat#20021
High-Capacity cDNA Reverse Transcription Kit Thermo Fisher Cat#4368814
Deposited data
RNA sequencing reads NCBI Sequence Read Archive PRJNA803354
Experimental models: Organisms/strains
Mouse: C57BL/6J The Jackson Laboratory JAX: 000664
Mouse: NOD.CgPrkdcscid/J The Jackson Laboratory JAX: 001303
C. elegans: GC833: glp-1(ar202) III E. Jane Hubbard, (Pepper et al., 2003) N/A
C. elegans: GC1250: daf-16(m26) I; daf-2(e1370) glp-1(e2141) III E. Jane Hubbard, (Michaelson et al., 2010) N/A
C. elegans: GC1474: glp-1(e2141) III; hjSi20 [Pmyo-2P::mCherry::unc-54 3’UTR] IV; zuIs70 [pJN152: end-1P::gfp::caax; unc-119(+)] V this paper N/A
C. elegans: GC1545: daf-5(e1386) II; glp-1(e2141) III this paper N/A
Oligonucleotides
See table S4.
Recombinant DNA: List of plasmids used in this study
pGC735, eutN cloned into pBR322 by Gibson assembly this paper
pGC737, pET28 this paper
pGC738, fabH cloned into pBR322 by Gibson assembly this paper
pPlac-eutD, eutD under the control of a lacUV5 promoter this paper
pBR322 Sigma-Aldrich Cat#OGS589
Sigma-Aldrich Cat#69864-3
Software and algorithms
Flowjo 10.0.8 Flowjo, LLC https://flowjo.com/
Illustrator CC Adobe https://www.adobe.com/products/illustrator.html
ImageJ https://imagej.nih.gov
ActivityBase IDBS https://www.idbs.com
Software: R v3.4.1 https://www.r-statistics.com/