Table 1.
DA potency, detection range, dynamic range, and kinetic parameters of DA sensors.
| Detection range | Dynamic range | Kinetic parameters | |||
|---|---|---|---|---|---|
| Sensor | pEC50 ± SEM (nM)a | 10–90% (nM)a | F/F0 ± SEMb | kon (M−1 min−1)a | koff (min−1)a |
| dLight1.1 | 6.45 ± 0.12 (350) | 40–3100 | 2.29 ± 0.06 | 1.84 ± 0.76 × 108 | 120 ± 17 |
| dLight1.2 | 5.92 ± 0.04 (1200) | 140–10,000 | 3.16 ± 0.20 | 7.50 ± 0.59 × 107 | 130 ± 17 |
| dLight1.3a | 5.87 ± 0.06 (1300) | 130–14,000 | 4.98 ± 0.24 | 6.42 ± 0.72 × 107 | 150 ± 4.8 |
| dLight1.3b | 5.67 ± 0.02 (2100) | 190–17,000 | 6.61 ± 0.47 | 7.17 ± 1.60 × 107 | 115 ± 10 |
| GRABDA1M | 7.12 ± 0.04 (75) | 4.0–1400 | 1.86 ± 0.07 | 6.60 ± 1.66 × 108 | 80 ± 6.0 |
| GRABDA1H | 8.34 ± 0.19 (4.6) | 0.78–27 | 2.49 ± 0.04 | ND | 9.2 ± 0.42 |
| GRABDA2M | 6.90 ± 0.07 (130) | 8.6–1800 | 4.77 ± 0.22 | 3.14 ± 0.38 × 108 | 45 ± 4.3 |
ND Not determine due to ligand depletion.
aDetermined with a fluorescent plate reader.
bDetermined with a fluorescent microscope.