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. 2022 Jun 10;12:9637. doi: 10.1038/s41598-022-14311-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Characterisation of a set of reference DR agonists (A) and antagonists (B) in the DMR assay. CHO-K1 hD_2longR cells were treated with varying concentrations of the indicated ligands and the DMR signal was recorded over a time-course of 60 min. The maximum wavelength shift (Δλ_max) was used to construct concentration-effect curves. In agonist mode (A), the response was normalised to a solvent control (0%) and the maximum response induced by 1 μM quinpirole (100%). In antagonist mode (B), the cells were preincubated with various concentrations of antagonist for 30 min before quinpirole was added at a concentration (30 nM) that induces the response equal to 80% of the maximal response induced by 1 μM of quinpirole. The response was normalised to a solvent control (0%) and the response induced by 30 nM quinpirole (100%). Data represent means ± SEM of three independent experiments, performed in triplicate.