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. 2022 Jun 10;12:9637. doi: 10.1038/s41598-022-14311-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of calcium depletion on the quinpirole-induced DMR traces of hD_2longR expressing CHO-K1 cells. (A) Response induced by quinpirole (1 μM) in the absence or in the presence of EGTA (2 mM) in the assay buffer. (B) Cells were pre-incubated with thapsigargin (1 μM or 2 μM) for 20 min before the addition of quinpirole (1 μM). EGTA (2 mM) was present in the assay buffer. (C) Thapsigargin-induced effect on CHO-K1 hD_2longR cells. The assay buffer was supplemented with EGTA (2 mM). In all experiments, after replacement of the medium by the EGTA-containing buffer, cells were allowed to condition in the pre-heated plate reader (28 °C) for 2 h. Data were normalised to the maximum change in wavelength shift induced by quinpirole (1 μM) observed in untreated CHO-K1 hD_2longR cells (100%) and a buffer control (0%). Data shown are means ± SEM of representative recordings performed in triplicate of three independent experiments.