TABLE 2.
Crude biosurfactant or compound | Elemental concn ratios
|
Fractions of carbon and oxygen involved in various chemical functions
|
|||||||
---|---|---|---|---|---|---|---|---|---|
N/C | O/C | P/C | C—(C,H) | C—(O,N) | C⩵O | O⩵C—OH | (O⩵C)/C | (OH)/C | |
Biosurfactant released by: | |||||||||
S. mitis BA | 0.172 | 0.407 | 0.019 | 0.58 | 0.24 | 0.18 | 0 | 0.342 | 0.065 |
S. mitis BMS | 0.101 | 0.426 | 0.016 | 0.64 | 0.20 | 0.13 | 0.04 | 0.268 | 0.158 |
Acid-precipitated S. mitis BMS biosurfactant | 0.071 | 0.366 | 0.016 | 0.61 | 0.24 | 0.11 | 0.05 | 0.121 | 0.244 |
Reference compounds | |||||||||
Proteina | 0.270 | 0.32 | 0 | 0.41 | 0.32 | 0.28f | 0.275 | 0.045 | |
Glycosidic residueb | 0 | 0.83 | 0 | 0 | 0.83 | 0.17f | 0 | 0.833 | |
Rhamnolipid R1c | 0 | 0.406 | 0 | 0.56 | 0.31 | 0.06 | 0.06 | 0.061 | 0.345 |
Rhamnolipid R2c | 0 | 0.346 | 0 | 0.65 | 0.23 | 0.04 | 0.08 | 0.076 | 0.269 |
Phospholipidd | 0.009 | 0.21 | 0.034 | 0.82 | 0.13 | 0.05 | 0.01 | 0.077 | 0.132 |
LTAe | 0.031 | 0.63 | 0.066 | 0.41 | 0.44 | 0.44 | 0.10 | 0.151 | 0.478 |
Average protein, calculated based on a collection of bacterial, fungal, and mammalian proteins (21).
Glycosidic residue C6H10O5 (21).
Calculated for rhamnolipids synthesized by Pseudomonas aeruginosa. R1 contains two rhamnose units, and R2 contains one rhamnose unit (6, 11).
l-α-Phosphatidyl-dl-glycerol dimyristoyl.
LTA, Lipoteichoic acid from S. mutans (Sigma).
C⩵O and O⩵C—OH together.