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. 2022 Jun 10;12:9605. doi: 10.1038/s41598-022-13724-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Mean CB1R intensity within excitatory (vGlut1-IR +) and inhibitory (vGAT-IR +) cell types across three brain regions of the monkey cortex. Each individual data point represents mean intensity averaged across all sampled sites across a cortical layer. There was a main effect of cell type, p < 0.001, main effect of brain region, p < 0.001, and a significant cell type × brain region interaction, p < 0.001. While there were no differences in mean CB1R intensity within excitatory populations across brain regions, post hoc analysis indicated mean CB1R intensity within inhibitory populations was highest in the primary auditory cortex, followed by the association auditory cortex, with lowest mean CB1R intensity within inhibitory populations in the PFC, p < 0.001. Straight lines indicate main effects across groups. Brackets indicate post hoc pairwise comparisons. **p < 0.001. Error bar denotes SEM. PFC = prefrontal cortex, A1 = primary auditory cortex, A2 = association auditory cortex.