Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jun 10;13:3349. doi: 10.1038/s41467-022-31082-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — a Diagram of hM4Di transduction in vCA3 and cannulation in LScd, with b representative DREADDs axonal transduction in the LScd (red) and somatic transduction in vCA3, c cannula placements in the LScd restricted to sections +0.7 to +0.2 anterior to bregma⁷¹, d minimum and maximum DREADDs expression in vCA3 observed in sections -5.20 to -5.80 posterior to bregma⁷¹. e Diagram of hM4Di transduction in vCA1 and cannulation in LSrv, with f representative DREADDs axonal transduction in LSrv and somatic transduction in vCA1, g cannula placement in the LSrv restricted to sections +1.2 to +0.48 anterior to bregma⁷¹, and h minimum and maximum DREADDs expression in vCA1, observed in sections -4.80 to -6.72 posterior to bregma⁷¹ i, l Representative images of c-Fos expression in the LScd and LSrv of hM4Di-expressing rats following either saline or CNO infusion. j, k Intracerebral CNO infusions attenuated c-Fos expression in areas of the LS that receive hM4Di positive inputs (Drug x Virus: F_(1,38) = 5.17, p = 0.0248; post hoc CA1 hM4Di t_7.16 = 3.49, p = 0.0022; post hoc CA3 hM4Di t_7.21 = 3.64, p = 0.0228; Region: F_(1,38) = 25.73, p = 0.0001) compared to saline controls. EGFP-LScd-CNO n = 5, EGFP -LScd-Saline n = 5, EGFP -LSrv-CNO n = 5, EGFP-LSrv-Saline n = 6. hM4Di-LScd-CNO n = 6, hM4Di-LScd-Saline n = 6, hM4Di-LSrv-CNO n = 7, hM4Di-LSrv-Saline n = 6. c-Fos analyzed by three-way ANOVA, followed by post hoc two-sided t-tests with Bonferroni-Holm correction for multiple comparisons. Data represent mean ± sem. *p < 0.05. * denotes between-subject comparisons. 100 um scale bars for b, f (left), i, l 1 mm scale bar for images b, f (right), c, g. i Cannula track shown by white vertical bar for images in c and d. dHPC dorsal hippocampus, LScd caudodorsal lateral septum, LSrv rostroventral lateral septum, vHPC ventral hippocampus. Source data are provided as a Source Data file.