Fig. 3.
Acetylated tubulin (Act-Tubulin) immunoreactivity in veliger larvae of Crepidula fornicata and Crepidula atrasolea. Green–Cilia, Acetylated Tubulin; Gray–Nuclei, DAPI. (A–C) Different views of a veliger larva of C. fornicata, with Act-tubulin immunoreactivity primarily found in cilia of the velum and the mantle. (D–F) Acetylated tubulin immunoreactivity in a veliger larva of C. atrasolea. Acetylated tubulin immunoreactivity is present in cilia of the velum and mantle edge. Note the differences in larval features between the two species, especially in regards to a reduced larval velum, presence of left and right tentacles, pronounced mantle cavity, and difference in overall veliger size in C. atrasolea compared to C. fornicata.