MEF2D promotes YAP activation and the proliferation of HCC cells. A. The expression levels of MEF2D, total YAP and phosphorylated YAP in HepG2, Huh7, and PLC/PRF/5 cells were detected by western blot. B. The relative ratios of phosphorylated and total YAP protein were determined in the above HCC cells. The data represent the means ± SDs of triplicate experiments. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. C. The absorbance of Huh7 cells transfected with MEF2D or GFP vector were determined by CCK8 assay at the indicated time points. D. The absorbance of Huh7 cells transfected with shMEF2D or scrambled vector were also determined by CCK8 assay. E. The cell viability results of the indicated groups at 24 hours were tested by CCK8 experiment in HepG2 cells. The above experimental results were obtained through three independent experiments. The data represent the means ± SDs of triplicate experiments. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.