Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jun 11;20:272. doi: 10.1186/s12951-022-01452-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 3 — Cell viability, cell uptake and protective effect of QU-Nanogel on A549 cells or RAW264.7 cells. Cell viability of A549 cells treated with different drugs by MTS assay. (a) Incubated in different concentrations of PQ for 6 and 12 h. (b) Incubated in quercetin, QU-Nanogel, BLK-Nanogel and ulinastatin for 6 and 12 h. (**) denotes statistical significance in comparison to the control group (**p < 0.01). (c) The uptake of QU-Nanogel by RAW264.7 cells were analyzed by inverted fluorescence microscope. QU-Nanogel was labeled with rhodamine B isothiocyanate (RBITC) (Red fluorescence), DAPI (Blue fluorescence) and DiO (Green fluorescence) was used to stain the cell nuclei and cell menbrance. (d) The protective effects of quercetin and QU-Nanogel on PQ (600 µM) injured A549-GFP cells. The A549-GFP cells emitted bright green fluorescence in the state of cell survival. DiI (Red fluorescence) and DAPI (Blue fluorescence) were used to dye cell membrane and cell nucleus, respectively