Figure 4.

Effect of BMP7 on the proinflammatory cytokine production. Orbital fibroblasts obtained from GO patient (n = 3) and normal control non-GO subjects (n = 3) were treated with IL-β (10 ng/mL, 24 hours) or TNF-α (10 ng/mL, 24 hours) with or without pretreatment with 100 ng/mL rhBMP7 for one hour. (A, B) Western blot analyses were performed to compare protein expressions of the proinflammatory cytokines, IL-6, IL-8, and ICAM-1. Experiments were performed in three GO and three non-GO cells from different individuals. The representative gel images are shown. (C, D) Quantification was performed using densitometry. The results are presented as the mean density ratio ± SD, normalized to the level of β-actin in the same sample (*P < 0.05).