Detection of 238-bp dioxygenase amplicons by PCR and hybridization using primers DEG-F and DEG-R. Lane M, 100- to 3,000-bp marker (Bio-Rad); lanes 1 to 8, P. putida HS1, P. putida mt-2, P. aeruginosa JI104, Pseudomonas sp. strain IC, P. putida P35X, Pseudomonas sp. strain CF600, P. putida H, and Pseudomonas sp. strain PpG7, respectively. Agarose gel (1.5% agarose) of the amplicons with approximately equal amounts of DNA in each lane; (B, C, and D) membranes hybridized under high (B)-, medium (C)-, and low (D)-stringency conditions using an exposure time of 2 to 3 h.