Competitive quantitative PCR performed on Pseudomonas sp. strain PpG7 cells using primers DEG-F and DEG-R. Lane M, 100- to 3,000-bp marker; lanes 1 to 6, 1 ng of target DNA (each lane) plus 1.1 × 108, 1.1 × 107, 1.1 × 106, 1.1 × 105, 1.1 × 104, and 1.1 × 103 copies of competitor per reaction mixture, respectively. Lane 7 is a control (no DNA) lane.